RP-HPLC determination of chlorogenic acid in Lycium barbarum L. extract and its protective effect on retinal ganglion cells
نویسندگان
چکیده
To determine the chlorogenic acid content in Lycium barbarum L. by HPLC (Reversed Phase Highperformance Liquid Chromatography); and to observe the protective effect of Lycium barbarum L. extract on Retinal Ganglion Cells (RGCs) in rats with ocular hypertension. A rat model of chronic ocular hypertension was established using healthy adult rats. The rats were randomized into the model group and the treatment group. The effects of Lycium barbarum L. extract on Intraocular Pressure (IOP), multifocal Electroretinogram (mfERG), apoptosis of RGCs and retinal Nitric Oxide (NO) content of rats with ocular hypertension were observed. The average recovery of chlorogenic acid was 100.1%, RSD=0.9%. Chlorogenic acid content in Lycium barbarum L. extract was 40.71 mg/g. Lycium barbarum L. extract could effectively prevent the elevation of IOP; improve the rat mfERG; significantly lower the retinal NO content in rats; and reduce the apoptosis of RGCs. Lycium barbarum L. extract has a protective effect on RGCs in rats with ocular hypertension. The proposed HPLC method is simple, accurate and reproducible, which can be used as the mass control standard of Lycium barbarum L. extract.
منابع مشابه
Modulation of microglia by Wolfberry on the survival of retinal ganglion cells in a rat ocular hypertension model
The active component of Wolfberry (Lycium barbarum), lycium barbarum polysaccharides (LBP), has been shown to be neuroprotective to retinal ganglion cells (RGCs) against ocular hypertension (OH). Aiming to study whether this neuroprotection is mediated via modulating immune cells in the retina, we used multiphoton confocal microscopy to investigate morphological changes of microglia in whole-mo...
متن کاملPolyphenolic content, antioxidant and antimicrobial activities of Lycium barbarum L. and Lycium chinense Mill. leaves.
This study was performed to evaluate the in vitro antioxidant and antimicrobial activities and the polyphenolic content of Lycium barbarum L. and L. chinense Mill. leaves. The different leave extracts contain important amounts of flavonoids (43.73 ± 1.43 and 61.65 ± 0.95 mg/g, respectively) and showed relevant antioxidant activity, as witnessed by the quoted methods. Qualitative and quantitativ...
متن کاملProtection of Retinal Ganglion Cells and Retinal Vasculature by Lycium Barbarum Polysaccharides in a Mouse Model of Acute Ocular Hypertension
Acute ocular hypertension (AOH) is a condition found in acute glaucoma. The purpose of this study is to investigate the protective effect of Lycium barbarum polysaccharides (LBP) and its protective mechanisms in the AOH insult. LBP has been shown to exhibit neuroprotective effect in the chronic ocular hypertension (COH) experiments. AOH mouse model was induced in unilateral eye for one hour by ...
متن کاملLycium barbarum polysaccharides protected human retinal pigment epithelial cells against oxidative stress-induced apoptosis.
AIM To investigate the protective effect and its mechanism of lycium barbarum polysaccharides (LBP) against oxidative stress-induced apoptosis in human retinal pigment epithelial cells. METHODS ARPE-19 cells, a human retinal pigment epithelial cell lines, were exposed to different concentrations of H2O2 for 24h, then cell viability was measured by Cell Counting Kit-8 (CCK-8) assay to get the ...
متن کاملActivation of the Nrf2/HO-1 Antioxidant Pathway Contributes to the Protective Effects of Lycium Barbarum Polysaccharides in the Rodent Retina after Ischemia-Reperfusion-Induced Damage
Lycium barbarum polysaccharides (LBP), extracts from the wolfberries, are protective to retina after ischemia-reperfusion (I/R). The antioxidant response element (ARE)-mediated antioxidant pathway plays an important role in maintaining the redox status of the retina. Heme oxygenase-1 (HO-1), combined with potent AREs in its promoter, is a highly effective therapeutic target for the protection a...
متن کامل